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Choosing the right pipette tip makes the experiment more efficient
发布时间:2025-08-26 13:46:34

Pipette tips are commonly used consumables in laboratories. They are used in conjunction with pipettes to precisely transfer small amounts of liquid. Choosing the right pipette tip is crucial for enhancing experimental efficiency and ensuring the accuracy of experimental results. Here are some key points to consider when choosing pipette tips

Range matching: When choosing a pipette tip, make sure its range matches the volume of the liquid to be transferred. Generally speaking, the volume of pipetting should be within the range of 35% to 100% of the pipette tip's capacity. Within this range, the accuracy and repeatability of the pipette tip perform at their best. Avoid using small-range pipettes to transfer large amounts of liquid or large-range pipettes to transfer trace amounts of liquid, as this may cause significant errors and affect the accuracy of the experimental results.

Material and compatibility: Common pipette tip materials include polypropylene (PP), polyethylene (PE), etc. These materials usually have good chemical stability and biocompatibility, and can meet the needs of most routine experiments. At the same time, the compatibility between the pipette tip and the pipette should be taken into consideration to ensure that the two can fit closely and seal well to prevent liquid leakage. Otherwise, it may lead to inaccurate pipetting volume and even affect the normal progress of the experiment.

Special Requirements: If the experiment involves transferring samples containing viscous, high-concentration or corrosive liquids, pipette tips with special coatings or designs should be selected to reduce liquid residue and adsorption, and improve pipetting efficiency. For instance, for high-viscosity liquids, low-adsorption pipette tips can be selected; For volatile and corrosive chemicals, pipette tips with filter elements can be used to prevent liquid evaporation or corrosion of the pipette, and also to avoid cross-contamination. If experiments with high sensitivity requirements are being conducted, such as PCR and DNA sequencing, or when handling precious samples or reagents, to reduce sample loss and contamination, low-adsorption tips are a better choice. In addition, in aseptic operating environments, such as cell culture experiments, sterile pipette tips need to be used to prevent microbial contamination.


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